Restricted-access Media: Solid-phase Extraction

reversed-phase (ISRP) material. Proteins do not adsorb on the hydrophilic exterior surfaces and do not penetrate into the hydrophobic interior surfaces, while analytes can reach the interior surfaces and be separated. (Reproduced with permission from Perry JA (1991) The internal surface reversed phase. Concept and applications. Journal of Liquid Chromatography 13: 1047}1074.) Gelfferich F (1962) Ion-exchange. NewYork:McGraw-Hill. Graf H, Rabaud JN and Egly UM (1994) Ion-exchange resins for the puriRcation of monoclonal antibodies from animal cell culture. Bioseparation 4: 7}20. Greig JA (ed.) (1996) Ion-exchange Developments and Applications. Proceedings of IEX ’96. Cambridge: Royal Society of Chemistry. Levison PR (1993) Process scale liquid chromatography. In: Kennedy JF, Philips GO and Williams PA (eds) Cellulosics: Materials for Selective Separation and Other Technologies. Chichester: Ellis-Horwood. Pirotta M (1991) Ion-exchangers in pharmacy, medicine and biochemistry. In: Dorfner K (ed.) Ion Exchangers. New York. Rossomando EF (1990) Ion-exchange chromatography. In: Deutscher MP (ed.) Methods in Enzymology, vol. 182, Guide to Protein PuriTcation, pp. 309, 409. New York: Academic Press. Streat M and Cloete FLD (1987) Ion exchange. In: Rousseau RW (ed.)Handbook of Separation Process Technology. New York: Wiley.